基本上,GenBank文件包含基因条目('基因'后面跟着相应的' CDS'条目(每个基因只有一个),就像我在下面显示的两个一样。我想在制表符分隔的两列文件中获取locus_tag vs product。'基因' CDS'总是先后跟空格。如果这个任务可以很容易地使用一个已经可用的工具,请告诉我。
输入文件:
gene complement(8972..9094)
/locus_tag="HAPS_0004"
/db_xref="GeneID:7278619"
CDS complement(8972..9094)
/locus_tag="HAPS_0004"
/codon_start=1
/transl_table=11
/product="hypothetical protein"
/protein_id="YP_002474657.1"
/db_xref="GI:219870282"
/db_xref="GeneID:7278619"
/translation="MYYKALAHFLPTLSTMQNILSKSPLSLDFRLLFLAFIDKR"
gene 9632..11416
/gene="frdA"
/locus_tag="HAPS_0005"
/db_xref="GeneID:7278620"
CDS 9632..11416
/gene="frdA"
/locus_tag="HAPS_0005"
/note="part of four member fumarate reductase enzyme
complex FrdABCD which catalyzes the reduction of fumarate
to succinate during anaerobic respiration; FrdAB are the
catalytic subcomplex consisting of a flavoprotein subunit
and an iron-sulfur subunit, respectively; FrdCD are the
membrane components which interact with quinone and are
involved in electron transfer; the catalytic subunits are
similar to succinate dehydrogenase SdhAB"
/codon_start=1
/transl_table=11
/product="fumarate reductase flavoprotein subunit"
/protein_id="YP_002474658.1"
/db_xref="GI:219870283"
/db_xref="GeneID:7278620"
/translation="MQTVNVDVAIVGAGGGGLRAAIAAAEANPNLKIALISKVYPMRS
HTVAAEGGAAAVAKEEDSYDKHFHDTVAGGDWLCEQDVVEYFVEHSPVEMTQLERWGC
PWSRKADGDVNVRRFGGMKIERTWFAADKTGFHLLHTLFQTSIKYPQIIRFDEHFVVD
ILVDDGQVRGCVAMNMMEGTFVQINANAVVIATGGGCRAYRFNTNGGIVTGDGLSMAY
RHGVPLRDMEFVQYHPTGLPNTGILMTEGCRGEGGILVNKDGYRYLQDYGLGPETPVG
KPENKYMELGPRDKVSQAFWQEWRKGNTLKTAKGVDVVHLDLRHLGEKYLHERLPFIC
ELAQAYEGVDPAKAPIPVRPVVHYTMGGIEVDQHAETCIKGLFAVGECASSGLHGANR
LGSNSLAELVVFGKVAGEMAAKRAVEATARNQAVIDAQAKDVLERVYALARQEGEESW
SQIRNEMGDSMEEGCGIYRTQESMEKTVAKIAELKERYKRIKVKDSSSVFNTDLLYKI
ELGYILDVAQSISSSAVERKESRGAHQRLDYVERDDVNYLKHTLAFYNADGTPTIKYS
DVKITKSQPAKRVYGAEAEAQEAAAKKE"
所需的输出(locus_tag与制表符分隔的两列文件中的产品):
HAPS_0004 hypothetical protein
HAPS_0005 fumarate reductase flavoprotein subunit
事实上,这个输出是理想的,每个基因一行(仅显示一个基因):
locus_tag="HAPS_0004" db_xref="GeneID:7278619" complement(8972..9094) codon_start=1 transl_table=11 product="hypothetical protein" protein_id="YP_002474657.1" db_xref="GI:219870282" db_xref="GeneID:7278619" translation="MYYKALAHFLPTLSTMQNILSKSPLSLDFRLLFLAFIDKR"
答案 0 :(得分:3)
perl -nE'
BEGIN{ ($/, $") = ("CDS", "\t") }
say "@r[0,1]" if @r= m!/(?:locus_tag|product)="(.+?)"!g and @r>1
' file
输出
HAPS_0004 hypothetical protein
HAPS_0005 fumarate reductase flavoprotein subunit