很抱歉,我问了一个愚蠢的问题,但我自己找不到。。。我前一阵子学会了perl,我有点迷茫。
我想转换这种输出:
Palindromes of: seq1
Sequence length is: 24
Start at position: 1
End at position: 24
Minimum length of Palindromes is: 6
Maximum length of Palindromes is: 12
Maximum gap between elements is: 6
Number of mismatches allowed in Palindrome: 0
Palindromes:
1 aaaaaaaaaaa 11
|||||||||||
24 ttttttttttt 14
Palindromes of: seq2
Sequence length is: 15
Start at position: 1
End at position: 15
Minimum length of Palindromes is: 6
Maximum length of Palindromes is: 12
Maximum gap between elements is: 6
Number of mismatches allowed in Palindrome: 0
Palindromes:
1 aaaaaac 7
|||||||
15 ttttttg 9
放入gff或bed文件:
seq1 1 24
seq2 1 15
我找到了一个可以执行此操作的perl模块:https://metacpan.org/pod/Bio::Tools::GFF
这是我的小脚本:
#!/usr/bin/perl
use strict;
use warnings 'all';
use Bio::Tools::EMBOSS::Palindrome;
use Bio::Tools::GFF;
my $filename = "truc.pal";
# a simple script to turn palindrome output into GFF3
my $parser = Bio::Tools::EMBOSS::Palindrome->new(-file => $filename);
my $out = Bio::Tools::GFF->new(-gff_version => 3,
-file => ">$filename.gff");
while( my $seq = $parser->next_seq ) {
for my $feat ( $seq->get_SeqFeatures ) {
$out->write_feature($feat);
}
}
这是结果:
##gff-version 3
seq1 palindrome similarity 14 24 . - 1 allowed_mismatches=0;end=24;maximum gap=6;maximum_length=12;minimum_length=6;seqlength=24;start=1
seq2 palindrome similarity 9 15 . - 1 allowed_mismatches=0;end=15;maximum gap=6;maximum_length=12;minimum_length=6;seqlength=15;start=1
问题是:我希望得到回文的开始和结束以及最后一行中的特定位置的结果。
我想要的例子:
##gff-version 3
seq1 palindrome similarity 1 24 . - 1 mismatches=0;gap_positions=11-14;gap_size=3
seq2 palindrome similarity 1 15 . - 1 mismatches=0;gap_positions=7-9;gap_size=2
谢谢。