Question

我正在使用ImageJ宏来批量处理图像。具体来说，我正在尝试在每个图像上运行一个名为Log3D的插件。出于某种原因，ImageJ宏不会等待命令完成运行，然后再运行脚本中的下一行。

有没有解决的办法？

谢谢！

我目前正在使用“ waitForUser”手动让ImageJ知道何时继续，但是这很烦人，并且当我一次处理数百张图像时没有用。

selectWindow(file + " - C=" + chFISH);
run("LoG 3D", "sigmax=1.5 sigmay=1.5 sigmaz=1.5 displaykernel=0 volume=1");
waitForUser("Proceed when Log3D is complete");
run("Invert", "stack");
run("16-bit");
print("Saving smFIHS channel to: " + output);
saveAs("Tiff", output + File.separator + file + ".chFISH"); 
close();

Answer 1

您可以先在所有图像上运行Log3D，然后再在新处理的图像上运行其余部分。

openPath = getDirectory("Choose Source Directory");
files = getFileList(openPath);
savePath = getDirectory("Choose Destination Directory");

for (timePoint = 0; timePoint < (files.length); timePoint++)
{
    if (indexOf(files[timePoint], chFISH) >= 0) //Screens file names for your variable
    {
        tempName = getTitle();
        open(openPath + files[timePoint]);
        run("LoG 3D", "sigmax=1.5 sigmay=1.5 sigmaz=1.5 displaykernel=0 volume=1");
        saveAs("Tiff", savePath + tempName);
        close();
    }
}

files = getFileList(savePath);

for (timePoint = 0; timePoint < (files.length); timePoint++)
{
    run("Invert", "stack");
    run("16-bit");
    print("Saving smFIHS channel to: " + savePath);
    saveAs("Tiff", savePath + File.separator + file + ".chFISH"); 
    close();
}

Answer 2

如果图像的文件大小一致，则可以使用“等待”命令，这会使ImageJ等待“ n”毫秒才能继续。

selectWindow(file + " - C=" + chFISH);
run("LoG 3D", "sigmax=1.5 sigmay=1.5 sigmaz=1.5 displaykernel=0 volume=1");
wait(30000); //Waits for 30 seconds
run("Invert", "stack");
run("16-bit");
print("Saving smFIHS channel to: " + output);
saveAs("Tiff", output + File.separator + file + ".chFISH"); 
close();

您必须确定插件在给定映像上运行需要多长时间，但是原则上这应该可以解决您的问题。

ImageJ宏不等待命令完成运行

2 个答案: