尝试在命令行上执行AMR预测时,收到以下错误消息。
mykrobe predict tb_sample_id tb -1 /home/TB/demo_input_file_for_M.tuberculosis_app.fastq
选择的物种是结核病(TB),而样本数据文件是从Mykrobe网站(https://github.com/iqbal-lab/Mykrobe-predictor/releases/download/v0.1.1-beta/demo_input_file_for_M.tuberculosis_app.fastq.gz)下载的。
以及错误消息我正在......
Traceback (most recent call last):
File "/usr/local/bin/mykrobe", line 9, in <module>
load_entry_point('mykrobe==0.4.2', 'console_scripts', 'mykrobe')()
File "/usr/local/lib/python2.7/site-packages/mykrobe/mykrobe_predictor.py", line 99, in main
args.func(parser, args)
File "/usr/local/lib/python2.7/site-packages/mykrobe/mykrobe_predictor.py", line 32, in run_subtool
run(parser, args)
File "/usr/local/lib/python2.7/site-packages/mykrobe/cmds/amr.py", line 127, in run
cp.run()
File "/usr/local/lib/python2.7/site-packages/mykatlas/typing/typer/genotyper.py", line 73, in run
self._run_cortex()
File "/usr/local/lib/python2.7/site-packages/mykatlas/typing/typer/genotyper.py", line 90, in _run_cortex
self.mc_cortex_runner.run()
File "/usr/local/lib/python2.7/site-packages/mykatlas/cortex/mccortex.py", line 161, in run
self._run_cortex()
File "/usr/local/lib/python2.7/site-packages/mykatlas/cortex/mccortex.py", line 173, in _run_cortex
self._build_panel_binary_if_required()
File "/usr/local/lib/python2.7/site-packages/mykatlas/cortex/mccortex.py", line 190, in _build_panel_binary_if_required
subprocess.check_output(cmd)
File "/usr/local/lib/python2.7/subprocess.py", line 566, in check_output
process = Popen(stdout=PIPE, *popenargs, **kwargs)
File "/usr/local/lib/python2.7/subprocess.py", line 710, in __init__
errread, errwrite)
File "/usr/local/lib/python2.7/subprocess.py", line 1335, in _execute_child
raise child_exception
OSError: [Errno 2] No such file or directory
任何建议都将不胜感激。
答案 0 :(得分:1)
错误表示缺少mccortex31
。
您是否根据documentation安装了它?
cd Mykrobe-predictor
cd mccortex
make
export PATH=$PATH:$(pwd)/bin
cd ..
答案 1 :(得分:0)
我是Mykrobe预测者的作者之一。正如上面评论过的人,在我们的Mykrobe预测器存储库中,提出这个问题的正确位置可能是一个Github问题:
https://github.com/iqbal-lab/Mykrobe-predictor/issues
请随时提出问题。