R gplots中的heatmap.2出错

时间:2015-05-29 11:02:08

标签: r heatmap gplots

我在gplots中使用heatmap.2在R中创建热图。我的代码用于创建热图。但最近它开始抛出错误。我不确定为什么会这样。

# Matrix to feed heatmap.2
mat = as.matrix(sort_pkmat)
# color palette for diff. colors
my_palette <- colorRampPalette(c("white","red","red4"))(n = 299)
# color breaks for range
col_breaks = c(seq(0,40,length=100),
               seq(40,60,length=100),
               seq(60,90,length=100))

path1 = paste(path,name,'.png', sep = '')
print(path1)
png(path1, 
    width = 10*300,        # 5 x 300 pixels
    height = 8*300,
    res = 300,            # 300 pixels per inch
    pointsize = 8)


heatmap.2(mat, 
          #cellnote = mat,  # same data set for cell labels
          main = "Tag_density_HeatMap", # heat map title
          xlab = "Peaks",
          ylab = "Chip_samples",
          labRow = FALSE,
          labCol = FALSE,
          cexRow = 0.7,           # Changes the size of col and row font size
          cexCol = 0.2,
          notecol="black",      # change font color of cell labels to black
          density.info="none",  # turns off density plot inside color legend
          trace="none",         # turns off trace lines inside the heat map
          margins =c(3,5),     # widens margins around plot
          col=my_palette,       # use on color palette defined earlier
          breaks=col_breaks,    # enable color transition at specified limits
          dendrogram="none",     # only draw a row dendrogram
          Colv= FALSE,           #cluster column
          Rowv = FALSE,
          #keysize = 1
)
dev.off()

现在它抛出错误:

Error in seq.default(min.raw, max.raw, by = min(diff(breaks)/4)) : 
  invalid (to - from)/by in seq(.)
> dev.off()
null device 
          1 

此代码绘制热图,但不是颜色键

5 个答案:

答案 0 :(得分:2)

我使用heatmap.2遇到同样的问题。它似乎是由diff(break)返回的向量中有一个或多个零值产生的。当min(diff(break))为零时,'by'调用失败。我将断点更改为非重叠,并且可以显示颜色键。

最初我有:break = c(seq(0,0.033,length = 25),seq(0.033,0.066,length = 26),seq(0.066,0.1,length = 25))

我将其更改为: 符= C(SEQ(0,0.033,长度= 25),SEQ(0.0331,0.066,长度= 26),SEQ(0.0661,0.1,长度= 25))

它似乎解决了这个问题,并允许我再次使用颜色键。

答案 1 :(得分:1)

我遇到了同样的问题。我能够用钥匙制作一个情节,但没有我喜欢的控制:

我删除了&#34;休息&#34;完全选项并使用col = redblue(64)并生成带有键的双色热图。

答案 2 :(得分:1)

我遇到了以下问题,得到了上面的例子:

x = matrix(runif(100^2, min=0, max=100), ncol=100)
my_palette <- colorRampPalette(c("red", "yellow", "green"))(n = 99) # 100 percent
col_breaks = c(seq(0,10,length=40),  
             seq(11,40,length=30),              
             seq(41,100,length=30))
hm <- heatmap.2(x,
           col=my_palette,       
           breaks=col_breaks,
           dendrogram="none",
           Colv="NA", Rowv="NA")    

生成此热图:

starting heatmap

但是如果我在矩阵中引入一个负数,然后修改向量中的中断以解决这个负数,即

x = matrix(runif(100^2, min=-1, max=100), ncol=100)
col_breaks = c(seq(-1,10,length=40),  
             seq(11,40,length=30),              
             seq(41,100,length=30))
hm <- heatmap.2(x,
           col=my_palette,       
           breaks=col_breaks,
           dendrogram="none",
           Colv="NA", Rowv="NA")  

密钥完全失败:

failed heatmap

this answer之后添加simkey=FALSE解决了此问题,并且在此示例中似乎可行:

hm <- heatmap.2(x,
           col=my_palette,       
           breaks=col_breaks,
           symkey=FALSE,
           dendrogram="none",
           Colv="NA", Rowv="NA")

如您所见:

correct heatmap

答案 3 :(得分:1)

实际上我也遇到过这个问题,需要结合以上两个答案来结束工作代码。

  1. col_breaks 数字不应重叠。
  2. col_breaks 应该从负数开始到正数,反之亦然。 示例:
col_breaks = c(seq(-3,-1,length=100),  # for red
  seq(-0.99,0.99,length=100),           # for yellow
  seq(1,3,length=100))             # for green

注意:调用 heatmap.2() 函数时不需要添加 simkey=FALSE

答案 4 :(得分:0)

使用相同的断点(在我的例子中分别为10和40两次)给出了这个错误,即:

my_palette <- colorRampPalette(c("red", "yellow", "green"))(n = 99) # 100 percent


col_breaks = c(seq(0,10,length=40),  
                 seq(10,40,length=30),              
                 seq(40,100,length=30))

带来了这个错误,而

my_palette <- colorRampPalette(c("red", "yellow", "green"))(n = 99) # 100 percent



col_breaks = c(seq(0,10,length=40),  
                 seq(11,40,length=30),              
                 seq(41,100,length=30))

解决了以下错误:

x = matrix(runif(100^2, min=0, max=100), ncol=100)
hm <- heatmap.2(x,
               col=my_palette,       
               breaks=col_breaks,
               dendrogram="none",
               Colv="NA", Rowv="NA")