Question

我打算运行mbin进行甲基化分析。但收到错误消息：h5py.h5py_warnings.H5pyDeprecationWarning: dataset.value has been deprecated. Use dataset[()] instead. 尝试了几次，同时尝试使用buildcontrols提取控件IPD。
环境：

mbin版本：1.1.1
Python版本：2.7.12
操作系统：centOS
在virtualenv下运行

我认为这也是由版本引起的。我试过的我在同时使用python 3和python 2的服务器上进行了尝试，并指定virtualenv使用python 2作为解释器。 mbin已成功安装，但buidlcontrols没有安装。

(virtualenv_for_mbin) [yx@localhost virtualenv_for_mbin]$ buildcontrols -i --procs=4 --control_pkl_name=control_means_190201.pkl /data1/yuxiao/userdata1/jobs/016/016504/data/aligned_reads.cmp.h5
2019-02-01 23:36:31 [INFO] Initiating dictionary of all possible motifs...
2019-02-01 23:36:31 [INFO]   - Adding 256 4-mer motifs...
2019-02-01 23:36:31 [INFO] Done: 256 possible contiguous motifs

2019-02-01 23:36:31 [INFO]   - Adding 1024 5-mer motifs...
2019-02-01 23:36:31 [INFO] Done: 1536 possible contiguous motifs

2019-02-01 23:36:31 [INFO]   - Adding 4096 6-mer motifs...
2019-02-01 23:36:32 [INFO] Done: 7680 possible contiguous motifs

2019-02-01 23:36:32 [INFO]   - Adding bipartite motifs to search space...
2019-02-01 23:36:33 [INFO] Done: 194560 possible bipartite motifs

2019-02-01 23:36:33 [INFO] 
2019-02-01 23:36:33 [INFO] Preparing to create new control data in ctrl_tmp
Traceback (most recent call last):
  File "/data2/Software/virtualenv_for_mbin/bin/buildcontrols", line 10, in <module>
    sys.exit(launch())
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/mbin/controls.py", line 20, in launch
    extract_controls(opts, control_aln_fn)
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/mbin/controls.py", line 40, in extract_controls
    opts           = controls.scan_WGA_aligns()
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/mbin/controls.py", line 352, in scan_WGA_aligns
    reader = openIndexedAlignmentFile(self.control_aln_fn)
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/pbcore/io/opener.py", line 52, in openIndexedAlignmentFile
    return CmpH5Reader(fname, sharedIndex=sharedIndex)
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/pbcore/io/align/CmpH5IO.py", line 729, in __init__
    self._loadAlignmentInfo(sharedIndex)
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/pbcore/io/align/CmpH5IO.py", line 745, in _loadAlignmentInfo
    rawAlignmentIndex = self.file["/AlnInfo/AlnIndex"].value
  File "h5py/_objects.pyx", line 54, in h5py._objects.with_phil.wrapper
  File "h5py/_objects.pyx", line 55, in h5py._objects.with_phil.wrapper
  File "/data2/Software/virtualenv_for_mbin/lib/python2.7/site-packages/h5py/_hl/dataset.py", line 313, in value
    "Use dataset[()] instead.", H5pyDeprecationWarning)
h5py.h5py_warnings.H5pyDeprecationWarning: dataset.value has been deprecated. Use dataset[()] instead.

我检查了python版本：

(virtualenv_for_mbin) [yx@localhost virtualenv_for_mbin]$ python -V
Python 3.6.5 :: Anaconda, Inc.

所以，我立即尝试运行一个新的virtualenv。

[yx@localhost Software]$ mkdir venv_tmp
[yx@localhost Software]$ cd venv_tmp/
[yx@localhost venv_tmp]$ virtualenv --python=/usr/local/bin/python2.7 .
Running virtualenv with interpreter /usr/local/bin/python2.7
New python executable in /data2/Software/venv_tmp/bin/python2.7
Also creating executable in /data2/Software/venv_tmp/bin/python
Installing setuptools, pip, wheel...
done.
[yx@localhost venv_tmp]$ . bin/activate
(venv_tmp) [yx@localhost venv_tmp]$ python -V
Python 3.6.5 :: Anaconda, Inc.

然后，我转到另一台服务器.....，仅安装了python 2.7。

(venv_for_mbin) [yuxiao@localhost data_tmp]$ buildcontrols -i --procs=4 --control_pkl_name=control_means_190202.pkl aligned_reads.cmp.h5
2019-02-02 11:06:16 [INFO] Initiating dictionary of all possible motifs...
2019-02-02 11:06:16 [INFO]   - Adding 256 4-mer motifs...
2019-02-02 11:06:16 [INFO] Done: 256 possible contiguous motifs

2019-02-02 11:06:16 [INFO]   - Adding 1024 5-mer motifs...
2019-02-02 11:06:16 [INFO] Done: 1536 possible contiguous motifs

2019-02-02 11:06:16 [INFO]   - Adding 4096 6-mer motifs...
2019-02-02 11:06:16 [INFO] Done: 7680 possible contiguous motifs

2019-02-02 11:06:16 [INFO]   - Adding bipartite motifs to search space...
2019-02-02 11:06:18 [INFO] Done: 194560 possible bipartite motifs

2019-02-02 11:06:18 [INFO] 
2019-02-02 11:06:18 [INFO] Preparing to create new control data in ctrl_tmp
Traceback (most recent call last):
  File "/data1/yuxiao/venv_for_mbin/bin/buildcontrols", line 10, in <module>
    sys.exit(launch())
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/mbin/controls.py", line 20, in launch
    extract_controls(opts, control_aln_fn)
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/mbin/controls.py", line 40, in extract_controls
    opts           = controls.scan_WGA_aligns()
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/mbin/controls.py", line 352, in scan_WGA_aligns
    reader = openIndexedAlignmentFile(self.control_aln_fn)
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/pbcore/io/opener.py", line 52, in openIndexedAlignmentFile
    return CmpH5Reader(fname, sharedIndex=sharedIndex)
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/pbcore/io/align/CmpH5IO.py", line 729, in __init__
    self._loadAlignmentInfo(sharedIndex)
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/pbcore/io/align/CmpH5IO.py", line 745, in _loadAlignmentInfo
    rawAlignmentIndex = self.file["/AlnInfo/AlnIndex"].value
  File "h5py/_objects.pyx", line 54, in h5py._objects.with_phil.wrapper
  File "h5py/_objects.pyx", line 55, in h5py._objects.with_phil.wrapper
  File "/data1/yuxiao/venv_for_mbin/lib/python2.7/site-packages/h5py/_hl/dataset.py", line 313, in value
    "Use dataset[()] instead.", H5pyDeprecationWarning)
h5py.h5py_warnings.H5pyDeprecationWarning: dataset.value has been deprecated. Use dataset[()] instead.
(venv_for_mbin) [yuxiao@localhost data_tmp]$ python -V
Python 2.7.12

仍然收到相同的错误消息。我在这里希望有人能帮助我。非常感谢。

Answer 1

正如您所说，您需要使用获取标量值的新方法。 .value语法仍然有效，但是谁想在Jupyter中看到粉红色警告？

该消息不是很直观，语法也不是。因此，我将在此处进行拼写-您有一个名为f：

的d5py.File。

>>> f = h5py.File('temp.h5')
>>> f['path/to/scalar'] = 5
>>> f['path/to/scalar'].value
(...)\site-packages\h5py\_hl\dataset.py:313: H5pyDeprecationWarning: dataset.value has been deprecated. Use dataset[()] instead. "Use dataset[()] instead.", H5pyDeprecationWarning)
5

“改为使用数据集[（）]”实际上是“使用索引（__getitem__）运算符，并将空的tuple（()）传递给它。因此，最后一行应该显示为：

>>> f['path/to/scalar'][()]
5

如果您觉得这是一种奇怪的，异国情调的表示法，那么我只能同情。

但这很有道理-标量值的numpy.shape实际上是()。

错误消息：h5py.h5py_warnings.H5pyDeprecationWarning：dataset.value已被弃用。请改用数据集[（）]

1 个答案: