Question

我正在尝试使用Subprocess模块尝试将命令行指令发送到命令提示符。这是相关代码：

def get_primers(self):
    if self.inputbool == False or self.outputbool == False:
        tkinter.messagebox.showinfo('AUTOPRIMER', 'No input file and/or output destination detected!')
    else:
        if self.parambool == False:
            outputlocation = '-output=' + '"' + self.output + 'primer3output.txt' + '"'
            cmd = ['primer3_core', outputlocation, self.input]
            cmd2 = 'primer3_core' + ' ' + outputlocation + ' ' + self.input
            print(cmd2)
            subprocess.call(cmd)
            tkinter.messagebox.showinfo('AUTOPRIMER', 'Please check output file for desired content. If it is incorrect, please alter settings to achieve desired output.')
        else: #parameters present
            outputlocation = '-output=' + '"' + self.output + 'primer3output.txt' + '"'
            p3filesettings = self.p3filestring + '"' + self.param + '"'
            cmd = ['primer3_core', p3filesettings, outputlocation, self.input]
            cmd2 = 'primer3_core' + ' ' + p3filesettings + ' ' + outputlocation + ' ' + self.input
            print(cmd2)
            subprocess.call(cmd)
            tkinter.messagebox.showinfo('AUTOPRIMER', 'Please check output file for desired content. If it is incorrect, please alter settings to achieve desired output.')

命令的一般格式是对应用程序本身的调用，输出文件路径和输入文件路径。如果用户希望添加一个设置，则可能存在包含其他设置的文件路径。

示例：

primer3_core -p3_settings_file="C:/Users/mqian/Desktop/CGIProject/autoprimercode/output/test files/testingsettingscopy.txt" -output="C:/Users/mqian/Desktop/CGIProject/autoprimercode/output/test files/10-3/test2primer3output.txt

在命令提示符下键入该命令可以正常运行程序，但是当我尝试将这些组件作为列表甚至整个字符串传递给subprocess.call（）函数时，得到的输出如下：

C:\Users\mqian\AppData\Local\Programs\Python\Python37-32\python.exe C:/Users/mqian/Desktop/CGIProject/autoprimercode/windowsversion/workingwindowsautoprimer.py
primer3_core -p3_settings_file="C:/Users/mqian/Desktop/CGIProject/autoprimercode/output/test files/testingsettingscopy.txt" -output="C:/Users/mqian/Desktop/CGIProject/autoprimercode/output/test files/10-3/test2primer3output.txt" "C:/Users/mqian/Desktop/CGIProject/autoprimercode/output/test files/primerflowinput"
Exception in Tkinter callback
Traceback (most recent call last):
  File "C:\Users\mqian\AppData\Local\Programs\Python\Python37-32\lib\tkinter\__init__.py", line 1702, in __call__
    return self.func(*args)
  File "C:/Users/mqian/Desktop/CGIProject/autoprimercode/windowsversion/workingwindowsautoprimer.py", line 77, in get_primers
    subprocess.Popen(cmd)
  File "C:\Users\mqian\AppData\Local\Programs\Python\Python37-32\lib\subprocess.py", line 756, in __init__
    restore_signals, start_new_session)
  File "C:\Users\mqian\AppData\Local\Programs\Python\Python37-32\lib\subprocess.py", line 1155, in _execute_child
    startupinfo)
FileNotFoundError: [WinError 2] The system cannot find the file specified

我尝试设置shell = True来查看是否会有所不同，现在即使将它添加到PATH中，它也无法将Primer3_core识别为有效命令。

'primer3_core' is not recognized as an internal or external command,
operable program or batch file.

当前，我的理论是Windows中命令提示符处理引号的方式可能是问题，因为它们是命令中的双引号，但我不确定。任何帮助都会很棒！

Subprocess.call无法正确将命令传递给命令行

0 个答案: