添加Application_Start后出现禁止错误

Question

我的ASP.NET MVC应用程序运行正常，直到我想在Global.asax的Application_Start（）中初始化几个应用程序变量。我开始得到“HTTP错误403.14 - 禁止 Web服务器配置为不列出此目录的内容。“添加后，

protected void Application_Start(object sender, EventArgs e)
{
    Application["xyz"] = "xyz";

}

的Web.config

    <?xml version="1.0" encoding="utf-8"?>
<configuration>
  <configSections>
    <section name="entityFramework" type="System.Data.Entity.Internal.ConfigFile.EntityFrameworkSection, EntityFramework, Version=6.0.0.0, Culture=neutral, PublicKeyToken=b77a5c561934e089" requirePermission="false" />
  </configSections>

  <connectionStrings>
      providerName="System.Data.SqlClient" />-->
    <add name="xyzDB" connectionString="Data Source=.\SQLEXPRESS; Database=xyz; Integrated Security=SSPI" providerName="System.Data.SqlClient" />    
  </connectionStrings>
  <appSettings>
    <add key="webpages:Version" value="3.0.0.0" />
    <add key="webpages:Enabled" value="false" />
    <add key="ClientValidationEnabled" value="true" />
    <add key="UnobtrusiveJavaScriptEnabled" value="true" />
  </appSettings>
  <system.web>
    <authentication mode="None" />
    <compilation debug="true" targetFramework="4.5" />
    <httpRuntime targetFramework="4.5" />
    <customErrors mode="On" defaultRedirect="~/Error">
      <error statusCode="404" redirect="~/Error/NotFound" />
    </customErrors>
  </system.web>
  <system.webServer>
    <modules>
      <remove name="FormsAuthentication" />
      <remove name="UrlRoutingModule-4.0" />
      <add name="UrlRoutingModule-4.0" type="System.Web.Routing.UrlRoutingModule" />
    </modules>
    <validation validateIntegratedModeConfiguration="false" />
        <directoryBrowse enabled="false" />

  </system.webServer>
  <runtime>
    <assemblyBinding xmlns="urn:schemas-microsoft-com:asm.v1">
      <dependentAssembly>
        <assemblyIdentity name="Microsoft.Owin.Security" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-3.0.1.0" newVersion="3.0.1.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="Microsoft.Owin.Security.OAuth" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-3.0.1.0" newVersion="3.0.1.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="Microsoft.Owin.Security.Cookies" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-3.0.1.0" newVersion="3.0.1.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="Microsoft.Owin" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-3.0.1.0" newVersion="3.0.1.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="Newtonsoft.Json" culture="neutral" publicKeyToken="30ad4fe6b2a6aeed" />
        <bindingRedirect oldVersion="0.0.0.0-9.0.0.0" newVersion="9.0.0.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="System.Web.Optimization" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="1.0.0.0-1.1.0.0" newVersion="1.1.0.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="WebGrease" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-1.5.2.14234" newVersion="1.5.2.14234" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="System.Web.Helpers" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="1.0.0.0-3.0.0.0" newVersion="3.0.0.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="System.Web.Mvc" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-5.2.3.0" newVersion="5.2.3.0" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="System.Web.WebPages" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-3.0.0.0" newVersion="3.0.0.0" />
      </dependentAssembly>
    </assemblyBinding>
  </runtime>
  <entityFramework>
    <defaultConnectionFactory type="System.Data.Entity.Infrastructure.SqlConnectionFactory, EntityFramework" />
    <providers>
      <provider invariantName="System.Data.SqlClient" type="System.Data.Entity.SqlServer.SqlProviderServices, EntityFramework.SqlServer" />
    </providers>
  </entityFramework>

</configuration>

为什么会出现此错误，如何纠正？