data<-sample_data
Temperature<-as.factor(data$Temperature)
#to change the orientation of the factor levels
data$ZT<-factor(data$ZT, levels = c( "T0", "T2", "T4", "T6", "T8", "T10", "T12", "T14", "T16", "T18", "T20", "T22") )
ZT<-data$ZT
#to change the orientation of the factor levels
data$Genotype<-factor(data$Genotype, levels =c("Scarlett","S42-IL107","S42-IL176"))
Genotype<-data$Genotype
co1 <-data$co1
SD<-data$co1_sd
ggplot(data, aes(x= ZT,y=co1, group=Temperature, shape=Temperature, colour=Temperature)) +
geom_errorbar(aes(ymin=co1-SD, ymax=co1+SD), width=.7, size=.7)+
geom_line(aes(colour=Temperature), size=1.1)+
geom_point(aes(shape=Temperature),size=4)+
scale_color_manual(values=c("turquoise3","indianred2"))+
ylab("HvCO1 normalised expression")+
xlab(NULL)+
facet_wrap(~ Genotype,)
我正在使用此脚本在ggplot2中创建刻面图,有时脚本工作正常,但通常会发生数据点在一个方面或方面之间的因子级别之间混乱。此时标准误差条被改组而不是数据点。谁能告诉我为什么会这样?
这里我将它与excel图表在同一数据上进行了比较。方面2和3的误差条。
structure(list(Genotype = structure(c(3L, 3L, 3L, 3L, 3L, 3L,
3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L, 3L,
3L, 3L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L,
2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L, 1L,
1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L,
1L, 1L), .Label = c("S42-IL107", "S42-IL176", "Scarlett"), class = "factor"),
Temperature = structure(c(1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L,
1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L,
2L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L,
2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L,
1L, 1L, 1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L,
2L, 2L, 2L, 2L), .Label = c("20°C", "28°C"), class = "factor"),
ZT = structure(c(1L, 7L, 10L, 11L, 12L, 2L, 3L, 4L, 5L, 6L,
8L, 9L, 1L, 7L, 10L, 11L, 12L, 2L, 3L, 4L, 5L, 6L, 8L, 9L,
1L, 7L, 10L, 11L, 12L, 2L, 3L, 4L, 5L, 6L, 8L, 9L, 5L, 6L,
8L, 9L, 1L, 7L, 10L, 11L, 12L, 2L, 3L, 4L, 1L, 7L, 10L, 11L,
12L, 2L, 3L, 4L, 5L, 6L, 8L, 9L, 1L, 7L, 10L, 11L, 12L, 2L,
3L, 4L, 5L, 6L, 8L, 9L), .Label = c("T0", "T10", "T12", "T14",
"T16", "T18", "T2", "T20", "T22", "T4", "T6", "T8"), class = "factor"),
co1 = c(0.004047961, 0.002880266, 0.001519785, 0.000667166,
0.000722255, 0.001466025, 0.004227897, 0.005888254, 0.007957774,
0.007576948, 0.005425386, 0.006893992, 0.005856547, 0.002997377,
0.001162904, 0.000576041, 0.000504455, 0.000508026, 0.001192632,
0.002094363, 0.005691455, 0.005918894, 0.007556918, 0.007736937,
0.007104176, 0.00584571, 0.002929043, 0.001618641, 0.002028418,
0.003404469, 0.010245418, 0.011297415, 0.009594296, 0.010923272,
0.01016621, 0.006077751, 0.005442749, 0.005538876, 0.006345497,
0.005632192, 0.007438102, 0.005501253, 0.002168485, 0.00111058,
0.000951645, 0.001391862, 0.001377207, 0.003435266, 0.00514792,
0.004080233, 0.002140552, 0.001426237, 0.002272299, 0.004213762,
0.008400302, 0.008992385, 0.006691724, 0.008404276, 0.007611373,
0.005460758, 0.001109314, 0.000732904, 0.000268885, 0.000244801,
0.000280516, 0.00077615, 0.001283453, 0.003493581, 0.004653499,
0.002507832, 0.002864612, 0.001727669), co1_sd = c(0.00029648,
0.000613192, 0.000221025, 0.000224196, 0.000223837, 0.00044702,
0.000706827, 0.001155351, 0.000232335, 0.00032364, 0.000602965,
0.001063606, 0.000923741, 0.000410751, 0.000150132, 0.000303137,
0.000104852, 0.000114863, 0.000569747, 0.00063052, 0.003203487,
0.001025025, 0.00301162, 0.003984255, 0.001973626, 0.001211777,
0.000226229, 0.000558652, 0.000615758, 0.000770557, 0.000956524,
0.001361853, 0.001474607, 0.002037219, 0.001628115, 0.002383452,
0.000147587, 0.000699657, 0.001651883, 0.00174892, 0.001190546,
0.000660793, 0.000635082, 0.000207585, 0.000226194, 0.000328652,
0.000640193, 0.001926859, 0.00069947, 0.000505254, 0.000178241,
0.000205647, 0.000348112, 0.000289324, 0.003999638, 0.001073981,
0.000260649, 0.001288045, 0.001187429, 0.000991558, 9.29063e-05,
4.65434e-05, 2.95757e-05, 5.16943e-05, 0.000144199, 0.000470742,
0.000250978, 0.000972389, 0.00049583, 0.000280404, 0.000529082,
0.000287902)), .Names = c("Genotype", "Temperature", "ZT",
"co1", "co1_sd"), class = "data.frame", row.names = c(NA, -72L
))