使用ggplot2在热图中没有正确显示图例

Question

我正在尝试根据示例代码here在ggplot2中使用geom_tile制作标准化读取丰度值的热图。我当前的代码生成了所需范围的热图，但由于某些原因，热图中只显示了7个范围中的4个，我无法弄清楚问题是什么。当我在原始链接中跟踪示例时，它工作正常，所以我必须在代码中更改错误。任何人都可以帮我识别我的代码中导致此错误的错误吗？ 我想要有以下配色方案：

   -Inf < value <= 0 -> white
   0 < value <=1 -> yellow
   1< value <=10 -> orange
   10< value <= 100 -> darkorange2
   100< value <= 1000 -> red
   1000 <value <= 10000 -> red3
   10000 < value <= 32000 -> red4

这是我的代码：

    #re-order the labels in the order of appearance in the data frame
    df$label <- factor(df$X1, as.character(df$X1))
    # make the cuts
    df$value1 <-cut(df$value,breaks=c(Inf,0,1,10,100,1000,10000,32000),right = T)
    ggplot(data =  df, aes(x = label, y = X2)) + geom_tile(aes(fill=value1), colour= "black") + scale_fill_manual(breaks=c("(-Inf,0]", "(0,1]", "(1,10]", "(10,100]", "(100,1000]", "(1000,10000]", "(10000,32000]"),values =c("white","yellow","orange","darkorange2","red","red3","red4"))

这里是我的数据预览（实际数据有228行，包含6个不同实验中38个ID的每百万值读数）：

    head(df)
                 X1      X2      value                                label      value1
      1 merged_read_17785-997_aka_156_aka_21 RPM.MT1  91.783028 merged_read_17785-997_aka_156_aka_21    (10,100]
      2 merged_read_133362-79_aka_156_aka_21 RPM.MT1   6.403467 merged_read_133362-79_aka_156_aka_21      (1,10]
      3 merged_read_147828-69_aka_156_aka_20 RPM.MT1   4.268978 merged_read_147828-69_aka_156_aka_20      (1,10]
      4 merged_read_162443-60_aka_156_aka_21 RPM.MT1   0.000000 merged_read_162443-60_aka_156_aka_21    (-Inf,0]
      5 merged_read_262156-32_aka_156_aka_21 RPM.MT1   5.691971 merged_read_262156-32_aka_156_aka_21      (1,10]
      6 merged_read_22905-759_aka_159_aka_21 RPM.MT1 140.164780 merged_read_22905-759_aka_159_aka_21 (100,1e+03]

以下是我从上述数据得到的情节：

Answer 1

我想我想出来了，如果我从scale_fill_manual中取出break参数，那么所有的图例都会显示出来：

 ggplot(data =  df, aes(x = label, y = X2)) + geom_tile(aes(fill=value1), colour= "black") + scale_fill_manual(values =c("white","yellow","orange","darkorange2","red","red3","red4"))