Question

我已经尝试通过调整similar question来解决我的问题。但是，我得到以下错误的URL或我想要执行此操作的文件。

trying URL 'http://cbio.mskcc.org/microrna_data/human_predictions_S_C_aug2010.txt.gz'
Content type 'application/x-gzip' length 65933953 bytes (62.9 Mb)
opened URL
downloaded 62.9 Mb

 Show Traceback

 Rerun with Debug
 Error in open.connection(file, "rt") : cannot open the connection In addition: Warning message:
In open.connection(file, "rt") :
  cannot open zip file 'D:....'

这是我试过的：

url_S_C <- "http://cbio.mskcc.org/microrna_data/human_predictions_S_C_aug2010.txt.gz"
tmpFile <- tempfile()
fileName <- gsub(".gz","",basename(url_S_C))
download.file(url_S_C, tmpFile)
data <- read.table(unz(tmpFile, fileName))
unlink(tmpFile)

也许someoe在这里可以帮助我为什么这个特定的文件不适合我？请注意，此文件是安静的大（62.9 Mb），但我无法使用类似问题的URL重现错误。

谢谢！

Answer 1

一些其他选项，基数为R：

url <- "http://cbio.mskcc.org/microrna_data/human_predictions_S_C_aug2010.txt.gz"
tmp <- tempfile()
##
download.file(url,tmp)
##
data <- read.csv(
  gzfile(tmp),
  sep="\t",
  header=TRUE,
  stringsAsFactors=FALSE)
names(data)[1] <- sub("X\\.","",names(data)[1])
##
R> head(data)
   mirbase_acc mirna_name gene_id gene_symbol transcript_id ext_transcript_id           mirna_alignment
1 MIMAT0000062 hsa-let-7a    5270    SERPINE2    uc002vnu.2         NM_006216   uuGAUAUGUUGGAUGAU-GGAGu
2 MIMAT0000062 hsa-let-7a  494188      FBXO47    uc002hrc.2      NM_001008777 uugaUA-UGUU--GGAUGAUGGAGu
3 MIMAT0000062 hsa-let-7a   80025       PANK2    uc002wkc.2         NM_153638   uugauaUGUUGG-AUGAUGGAgu
4 MIMAT0000062 hsa-let-7a   26036      ZNF451    uc003pdp.2          AK027074    uuGAUAUGUUGGAUGAUGGAGu
5 MIMAT0000062 hsa-let-7a     586       BCAT1    uc001rgd.3         NM_005504    uugaUAUGUUGGAUGAUGGAGu
6 MIMAT0000062 hsa-let-7a   22903       BTBD3    uc002wnz.2         NM_014962  uuGAUAUGUUGGAU-GAUGG-AGu
                  alignment            gene_alignment mirna_start mirna_end gene_start gene_end
1     | :|: ||:|| ||| ||||    aaCGGUGAAAUCU-CUAGCCUCu           2        21        495      516
2     || |||:  ::||||||||:  acaaAUCACAGUUUUUACUACCUUc           2        19        459      483
3         |::||: ||||||||     aauuucAUGACUGUACUACCUga           3        17         77       99
4      || || |   | |||||||     ccCUCUAGA---UUCUACCUCa           2        21       1282     1300
5        :|| |:   ||||||||     guagGUAAAGGAAACUACCUCa           2        19       6410     6431
6    || || ||| || ||||| ||   uaCUUUAAAACAUAUCUACCAUCu           2        21       2265     2288
              genome_coordinates conservation align_score seed_cat energy mirsvr_score
1 [hg19:2:224840068-224840089:-]       0.5684         122        0 -14.73      -0.7269
2  [hg19:17:37092945-37092969:-]       0.6464         140        0 -16.38      -0.1156
3    [hg19:20:3904018-3904040:+]       0.6522         139        0 -16.04      -0.2066
4   [hg19:6:56966300-56966318:+]       0.7627         144        7 -14.51      -0.8609
5  [hg19:12:24964511-24964532:-]       0.6775         150        7 -15.09      -0.2735
6  [hg19:20:11906579-11906602:+]       0.5740         131        0 -12.59      -0.2540

或者，如果您使用的是类Unix系统，则可以获取.txt文件（在R之外或使用R中的system或system2），如下所示：< / p>

[nathan@nrussell tmp]$ url="http://cbio.mskcc.org/microrna_data/human_predictions_S_C_aug2010.txt.gz"
[nathan@nrussell tmp]$ wget "$url" && gunzip human_predictions_S_C_aug2010.txt.gz

然后按照上面的步骤进行操作，在human_predictions_S_C_aug2010.txt和wget执行的任何地方阅读gunzip，

data <- read.csv(
  "~/tmp/human_predictions_S_C_aug2010.txt",
  stringsAsFactors=FALSE,
  header=TRUE,
  sep="\t")

就我而言。

Answer 2

您可以通过以下方式将文件中的数据读入R（在Windows上测试）：

library(stringr)
library(plyr)
library(dplyr)

 # download and extract file from web  

temp <- tempfile()
download.file("http://cbio.mskcc.org/microrna_data/human_predictions_S_C_aug2010.txt.gz", temp)
gzfile(temp, 'rt')
data <- read.csv(temp, 
                 stringsAsFactors = FALSE,
                 nrows = 20)
unlink(temp)

# column names

my_names <- 
  str_split(names(data), "\\.") %>% 
  unlist(.)

# toy example using only first 6 rows of dataset

mickey_mouse_data <- 
  head(data) %>% 
  unlist(.) %>% 
  str_split(., "\t") %>% 
  ldply(.)

names(mickey_mouse_data) <- my_names[-1]

tbl_df(mickey_mouse_data)

   mirbase_acc mirna_name gene_id gene_symbol transcript_id ext_transcript_id
1 MIMAT0000062 hsa-let-7a    5270    SERPINE2    uc002vnu.2         NM_006216
2 MIMAT0000062 hsa-let-7a  494188      FBXO47    uc002hrc.2      NM_001008777
3 MIMAT0000062 hsa-let-7a   80025       PANK2    uc002wkc.2         NM_153638
4 MIMAT0000062 hsa-let-7a   26036      ZNF451    uc003pdp.2          AK027074
5 MIMAT0000062 hsa-let-7a     586       BCAT1    uc001rgd.3         NM_005504
6 MIMAT0000062 hsa-let-7a   22903       BTBD3    uc002wnz.2         NM_014962
Variables not shown: mirna_alignment (chr), alignment (chr), gene_alignment (chr),
  mirna_start (chr), mirna_end (chr), gene_start (chr), gene_end (chr),
  genome_coordinates (chr), conservation (chr), align_score (chr), seed_cat (chr), energy
  (chr), mirsvr_score (chr)

使用R下载和提取.gz数据文件

2 个答案: