Question

我有一个XML格式的BLAST输出文件。它是22个查询序列，每个序列报告50个命中。我想提取所有50x22的点击量。这是我目前拥有的代码，但它只从第一个查询中提取50个匹配。

from Bio.Blast import NCBIXM
blast_records = NCBIXML.parse(result_handle)
blast_record = blast_records.next()

save_file = open("/Users/jonbra/Desktop/my_fasta_seq.fasta", 'w')

for alignment in blast_record.alignments:
    for hsp in alignment.hsps:
            save_file.write('>%s\n' % (alignment.title,))
save_file.close()

有人有任何关于提取所有点击的建议吗？我想我必须使用其他东西而不是路线。希望这很清楚。谢谢！

乔恩

Answer 1

这应该得到所有记录。与原作相比的新颖性是

for blast_record in blast_records

这是一个python习惯用法，用于遍历“类似列表”对象中的项目，例如blast_records（检查CBIXML module documentation表明parse（）确实返回迭代器）

from Bio.Blast import NCBIXM
blast_records = NCBIXML.parse(result_handle)

save_file = open("/Users/jonbra/Desktop/my_fasta_seq.fasta", 'w')

for blast_record in blast_records:
  for alignment in blast_record.alignments:
      for hsp in alignment.hsps:
            save_file.write('>%s\n' % (alignment.title,))
  #here possibly to output something to file, between each blast_record
save_file.close()

Answer 2

我使用此代码提取所有结果

from Bio.Blast import NCBIXML
for record in NCBIXML.parse(open("rpoD.xml")) :
    print "QUERY: %s" % record.query
    for align in record.alignments :
        print " MATCH: %s..." % align.title[:60]
        for hsp in align.hsps :
            print " HSP, e=%f, from position %i to %i" \
                % (hsp.expect, hsp.query_start, hsp.query_end)
            if hsp.align_length < 60 :
                 print "  Query: %s" % hsp.query
                 print "  Match: %s" % hsp.match
                 print "  Sbjct: %s" % hsp.sbjct
            else :
                 print "  Query: %s..." % hsp.query[:57]
                 print "  Match: %s..." % hsp.match[:57]
                 print "  Sbjct: %s..." % hsp.sbjct[:57]


print "Done"

或更少的细节

from Bio.Blast import NCBIXML
for record in NCBIXML.parse(open("NC_003197.xml")) :
    #We want to ignore any queries with no search results:
    if record.alignments :
        print "QUERY: %s..." % record.query[:60]
        for align in record.alignments :
            for hsp in align.hsps :
                print " %s HSP, e=%f, from position %i to %i" \
                % (align.hit_id, hsp.expect, hsp.query_start, hsp.query_end)
print "Done"

我用过这个网站

http://www2.warwick.ac.uk/fac/sci/moac/currentstudents/peter_cock/python/rpsblast/

BioPython：从Blast输出文件中提取序列ID

2 个答案: