我有这个制表符分隔的数据集,如下所示:
PITG_00022 start_codon 262407 262409 -
PITG_00022 stop_codon 260777 260779 -
PITG_00022 exon 260867 262409 -
PITG_00022 CDS 260867 262409 -
PITG_00022 exon 260777 260826 -
PITG_00022 CDS 260780 260826 -
PITG_00023 start_codon 273160 273162 +
PITG_00023 stop_codon 274778 274780 +
PITG_00023 exon 272998 273288 +
PITG_00023 CDS 273160 273288 +
PITG_00023 exon 273368 273652 +
PITG_00023 CDS 273368 273652 +
PITG_00023 exon 273729 273788 +
PITG_00023 CDS 273729 273788 +
PITG_00023 exon 273885 273958 +
PITG_00023 CDS 273885 273958 +
PITG_00023 exon 274022 274127 +
PITG_00023 CDS 274022 274127 +
PITG_00023 exon 274194 274346 +
这是我的代码,在这里非常优雅地发布Renumbering tab delimited data in two columns relative to contents of a third column我做了一些更改,但我得到的错误(见下文)在进行这些更改之前就已存在:
import numpy
import pandas
import pandas as pd
import sys
sys.stdout = open("outtry2.txt", "w")
data = pd.read_csv('pinfestans-edited2.csv', sep='\t')#,
#converters={'STRAND': lambda s: s[0]})
groups = data.groupby(['STRAND', 'GENE_ID'])
corrected = []
for (direction, gene_name), group in groups:
print direction,gene_name
if group.index[group.TYPE=='start_codon']:
start_exon = group.index[group.TYPE=='exon'][0]
if direction == '+':
group['POSA'] = 1 + abs(group.POS1 - group.POS1[start_exon])
group['POSB'] = 1 + abs(group.POS2 - group.POS1[start_exon])
else:
group['POSA'] = 1 - abs(group.POS2 - group.POS2[start_exon])
group['POSB'] = 1 - abs(group.POS1 - group.POS2[start_exon])
print group
corrected.append(group)
以下是输出示例:
GENE_ID TYPE POS1 POS2 STRAND POSA POSB
104 PITG_00021 start_codon 258927 258929 + 1 3
105 PITG_00021 stop_codon 260547 260549 + 1621 1623
106 PITG_00021 exon 258927 260549 + 1 1623
107 PITG_00021 CDS 258927 260546 + 1 1620
+ PITG_00023
GENE_ID TYPE POS1 POS2 STRAND POSA POSB
114 PITG_00023 start_codon 273160 273162 + 163 165
115 PITG_00023 stop_codon 274778 274780 + 1781 1783
116 PITG_00023 exon 272998 273288 + 1 291
117 PITG_00023 CDS 273160 273288 + 163 291
118 PITG_00023 exon 273368 273652 + 371 655
119 PITG_00023 CDS 273368 273652 + 371 655
120 PITG_00023 exon 273729 273788 + 732 791
121 PITG_00023 CDS 273729 273788 + 732 791
122 PITG_00023 exon 273885 273958 + 888 961
123 PITG_00023 CDS 273885 273958 + 888 961
124 PITG_00023 exon 274022 274127 + 1025 1130
125 PITG_00023 CDS 274022 274127 + 1025 1130
126 PITG_00023 exon 274194 274346 + 1197 1349
这是我运行一段时间后得到的错误:
>>>>Traceback (most recent call last):
File "C:\Users\Chris\Documents\I.S\Part-2\Convert_to_exonfile.py", line 51, in <module>
if group.index[group.TYPE=='start_codon']:
ValueError: The truth value of an array with more than one element is ambiguous. Use a.any() or a.all()
>>>>
现在我手工测试了一些计算,它似乎完全符合我的要求,只有STRAND列出的基因为+(请注意,样本输入中的PITG_00022基因是在输出中丢失)。我认为它首先在+ STRAND组中进行计算,但是一旦它到达 - STRAND组,就会出现错误。现在我读到了关于使用any()
和all()
的内容,以及关于在类似的数组valueError线程中使用set()
的帖子,但我无法弄清楚这将如何解决我的问题或者我将如何实现它。提前感谢任何帮助,谢谢。