下面是我当前拥有的代码。我是新手程序员，正在编写一个小程序，该程序将自动进行一些引物设计工作流程（生物学家/生物信息学家代表）。
我现在遇到的问题是，我对OOP与TKinter的工作方式缺乏了解。我已经阅读了许多stackoverflow帖子，还观看了youtube视频，并阅读了试图解释它的指南，但我仍然有些茫然。我目前的理解是，每个窗口应该是自己的对象，其上方的窗口为其父对象。我试图用我的程序做到这一点。 当前，我有两个类，AUTOPRIMER和BlastAPI。 AUTOPRIMER是主窗口。我在该窗口中创建了一个按钮，单击该按钮应会打开一个新窗口。据我了解，我为该窗口创建了一个名为BlastAPI的新对象，该对象处理程序的特定要求。我看到许多指南建议将父对象放在新对象的init中，但是从父对象到主对象再到args *，kwargs **，我看到了太多的初始化变化。什么时候合适？另外，当前堆栈跟踪会提供此反馈，因为它甚至无法正确编译。
Traceback (most recent call last):
File "/Users/Thunderpurtz/Desktop/CGIStuff/AUTOPRIMER/autoprimercode/test1.py", line 201, in <module>
autoprimer = AUTOPRIMER(root)
File "/Users/Thunderpurtz/Desktop/CGIStuff/AUTOPRIMER/autoprimercode/test1.py", line 105, in __init__
self.blast = BlastAPI(self)
File "/Users/Thunderpurtz/Desktop/CGIStuff/AUTOPRIMER/autoprimercode/test1.py", line 150, in __init__
eValueSetting = Entry(parent)
File "/anaconda3/lib/python3.6/tkinter/__init__.py", line 2673, in __init__
Widget.__init__(self, master, 'entry', cnf, kw)
File "/anaconda3/lib/python3.6/tkinter/__init__.py", line 2289, in __init__
BaseWidget._setup(self, master, cnf)
File "/anaconda3/lib/python3.6/tkinter/__init__.py", line 2259, in _setup
self.tk = master.tk
AttributeError: 'AUTOPRIMER' object has no attribute 'tk'
[Finished in 0.289s]
从根本上讲，我认为我对gui编程的理解并不扎实，因此，如果有人可以提供一些很好的见解，那么。如果这个问题有点笼统，我将很乐意在评论中阐明。
import subprocess
from tkinter import *
from tkinter.filedialog import *
import tkinter.messagebox
class AUTOPRIMER:
def __init__(self, master):
#some functions, their content is removed as i do not believe they are relevant
def button1():
pass
def button2():
pass
def button3():
pass
def getPrimers():
pass
def PrimerParser():
pass
def poolPrimers():
pass
self.master = master
self.input = ""
self.output = ""
self.param = ""
self.inputbool = False
self.outputbool = False
self.parambool = False
self.p3filestring = '-p3_settings_file='
self.blast = BlastAPI(self)
master.title("Complete Genomics Inc.")
########## WIDGETS ##########
entry_1 = Entry(master) #input
entry_2 = Entry(master) #output
entry_3 = Entry(master) #parameters
label_1 = Label(master, text="AUTOPRIMER")
button_1 = Button(master, text="Input Filepath: ", command=button1)
button_2 = Button(master, text="Output Filepath: ", command=button2)
button_3 = Button(master, text="Parameters Filepath: ", command=button3)
button_get = Button(master, text="Get Primers", command=getPrimers)
button_parse = Button(master, text="Parse Primers", command = PrimerParser)
button_pool = Button(master, text="Pool Primers", command=poolPrimers)
button_blast = Button(master, text="Blast Primers", command=self.blast)
button_quit = Button(master, text="Quit", command=master.destroy)
########## LAYOUT ##########
label_1.grid(row=0, columnspan=4) #grid doesnt take left right, it takes NSEW directions
button_1.grid(row=1, sticky=E, padx=1, pady=1)
button_2.grid(row=2, sticky=E, padx=1, pady=1)
button_3.grid(row=3, sticky=E, padx=1, pady=1)
button_get.grid(row=4)
button_parse.grid(row=4, sticky=W, column=1)
button_pool.grid(row=4, sticky=W, column=2)
button_blast.grid(row=4, sticky=W, column=3)
button_quit.grid(row=4, sticky=W, column=4)
entry_1.grid(row=1, column=1, sticky=W, padx=1, pady=1)
entry_2.grid(row=2, column=1, sticky=W, padx=1, pady=1)
entry_3.grid(row=3, column=1, sticky=W, padx=1, pady=1)
class BlastAPI:
#class that does blast alignment on primers
from Bio.Blast import NCBIWWW
from Bio.Blast import NCBIXML
def __init__(self, parent):
self.parent = parent
super(BlastAPI, self).__init__() #saw this on another stackoverflow don't truly understand what it means
eValueSetting = Entry(parent)
closeButton = Button(parent, text="Close", command=self.destroy)
inputButton = Button(parent, text="Input file", command=doNothing)
entryField = Entry(parent)
#layout
self.title('Complete Genomics Inc.')
def blastPrimers():
filename = askopenfilename()
with open(filename) as file:
string = file.read()
fasta = fasta_string
result_handle = NCBIWW.qblast("blastn", "nt", fasta)
with open("my_blast.xml", "w") as out_handle:
out_handle.write(result_handle.read())
result_handle.close()
result_handle = open('my_blast.xml')
blast_record = NCBIXML.parse(result_handle)
evalue = 1 #add make it a GUI alterable value blastPrimers
item = next(blast_record)
E_VALUE_THRESH = eValueSetting
while True:
with open('BlastResults.txt', w) as blast:
try:
for alignment in item.alignments:
for hsp in alignment.hsps:
if hsp.expect < E_VALUE_THRESH: #use this to determine if the result will be applicable / HAVE USER SET / default value?
blast.write("****Alignment****")
blast.write("sequence:", alignment.title)
blast.write("length:", alignment.length)
blast.write("e value:", hsp.expect)
blast.write(hsp.query[0:75] + "...")
blast.write(hsp.match[0:75] + "...")
blast.write(hsp.sbjct[0:75] + "...")
item = next(blast_record)
except StopIteration:
print("Done!")
break
root = Tk()
autoprimer = AUTOPRIMER(root)
root.mainloop()
谢谢大家。
答案 0 :(得分：0)
好，所以这里有很多工作要做。我想这些丢失的部分是您的主要代码的一部分，但是如果没有它们，就无法测试您的代码。我将尽我所能，并设置您的类以从需要的tkinter对象继承。通过您的BlastAPI类中的按钮命令判断，我假设该类应为Toplevel()窗口。
我已经对您的代码进行了一些更改，而没有Bio.Blast，我已经更改了一些我认为您可能需要做的事情。
import subprocess
import tkinter as tk # import tkinter as tk is good for compatibility and maintainability. Don't use *
from tkinter.filedialog import *
import tkinter.messagebox
from Bio.Blast import NCBIWWW
from Bio.Blast import NCBIXML
class AutoPrimer(tk.Tk): # Class names should normally use the CapWords convention.
def __init__(self):
#initialization
tk.Tk.__init__(self)
self.title("Complete Genomics Inc.")
self.input = ""
self.output = ""
self.param = ""
self.inputbool = False
self.outputbool = False
self.parambool = False
self.p3filestring = '-p3_settings_file='
self.entry_input = tk.Entry(self)
self.entry_output = tk.Entry(self)
self.entry_parameters = tk.Entry(self)
self.entry_input.grid(row=1, column=1, padx=1, pady=1, sticky="w")
self.entry_output.grid(row=2, column=1, padx=1, pady=1, sticky="w")
self.entry_parameters.grid(row=3, column=1, padx=1, pady=1, sticky="w")
self.label1 = tk.Label(self, text="AUTOPRIMER").grid(row=0, columnspan=4)
tk.Button(self, text="Input Filepath: ", command=lambda: self.button1).grid(row=1, padx=1, pady=1, sticky="e")
tk.Button(self, text="Output Filepath: ", command=lambda: self.button2).grid(row=2, padx=1, pady=1, sticky="e")
tk.Button(self, text="Parameters Filepath: ", command=lambda: self.button3).grid(row=3, padx=1, pady=1, sticky="e")
tk.Button(self, text="Get Primers",).grid(row=4)
tk.Button(self, text="Parse Primers",).grid(row=4, column=1, sticky="w")
tk.Button(self, text="Pool Primers",).grid(row=4, column=2, sticky="w")
tk.Button(self, text="Blast Primers", command=lambda: BlastAPI(self)).grid(row=4, column=3, sticky="w")
tk.Button(self, text="Quit", command=self.destroy).grid(row=4, column=4, sticky="w")
#CLASS METHODS
#Series of buttons methods that take in the filepath and displays it in the text widget to the user
def button1(self):
self.entry_input.delete(0,END)
ifp = askopenfilename()
self.setInput(ifp)
self.entry_input.insert(0, ifp)
self.setInputBool(True)
def button2(self):
self.entry_output.delete(0,END)
ofp = asksavefilename()
self.setOutput(ofp)
self.entry_output.insert(0, ofp)
self.setOutputBool(True)
def button3(self):
self.entry_parameters.delete(0,END)
pfp = askopenfilename()
self.entry_parameters.insert(0, pfp)
self.setParameterBool(True)
#Methods that rely on class attributes after using above buttons to set
def get_primers(self):
pass
def primer_parser(self):
pass
def pool_primers(self):
pass
#Setters and Getters
def setInput(self, value):
self.input = value
def setOutput(self, value):
self.output = value
def setParam(self, value):
self.param = value
def setInputBool(self, value):
self.inputbool = value
def setOutputBool(self, value):
self.outputbool = value
def setParameterBool(self, value):
self.parambool = value
class BlastAPI(tk.Toplevel):
def __init__(self, parent):
tk.Toplevel.__init__(self, parent)
self.title('Complete Genomics Inc.')
self.e_value_thresh = ""
self.e_value_setting = tk.Entry(self)
self.e_value_setting.pack() # Used pack here for quick testing. You will need to work on geometry yourself.
tk.Button(self, text="Close", command=self.destroy).pack()
tk.Button(self, text="Input file").pack()
self.entry_field = tk.Entry(self)
self.entry_field.pack()
def blast_primers(self): # Nothing is calling this function in your example.
filename = askopenfilename()
with open(filename) as file:
string = file.read() # string is not being used here.
fasta = string # No such var name in code.
result_handle = NCBIWWW.qblast("blastn", "nt", fasta) # This had a typo NCBIWW instead of NCBIWWW.
with open("my_blast.xml", "w") as out_handle:
out_handle.write(result_handle.read())
result_handle.close()
result_handle = open('my_blast.xml')
self.blast_record = NCBIXML.parse(result_handle)
evalue = 1 # Is not being used here.
self.item = next(self.blast_record)
self.e_value_thresh = self.e_value_setting.get()
self.blast_write_loop()
def blast_write_loop(self):
# I don't really like while loops and they have problems in event based GUI's.
# I don't think a while loop is needed here anyway.
with open('BlastResults.txt', 'w') as blast:
try:
for alignment in self.item.alignments:
for hsp in alignment.hsps:
if hsp.expect < self.e_value_thresh:
blast.write("****Alignment****")
blast.write("sequence:", alignment.title)
blast.write("length:", alignment.length)
blast.write("e value:", hsp.expect)
blast.write(hsp.query[0:75] + "...")
blast.write(hsp.match[0:75] + "...")
blast.write(hsp.sbjct[0:75] + "...")
self.item = next(self.blast_record)
except StopIteration:
print("Done!")
autoprimer = AutoPrimer()
autoprimer.mainloop()