我正在将基于C＃MVC / WebApi的Web应用程序部署到新平台（Windows 2012 R2 Standard - IIS 8.5）。 Web应用程序本身响应正常，核心代码没有错误，与数据库的连接等等。
当任何客户端通过Web浏览器访问Web应用程序时，无法显示所有静态文件（CSS / Font / JS / Image），将返回 500内部服务器错误状态代码这些文件。
但是，如果缩小CSS / JS文件并通过C＃捆绑功能捆绑这些请求，则会正确返回这些请求。字体和图像仍然无法显示。
验证了以下内容：
<?xml version="1.0" encoding="utf-8"?>
<!--
For more information on how to configure your ASP.NET application, please visit
http://go.microsoft.com/fwlink/?LinkId=301879
-->
<configuration>
<configSections>
<!-- For more information on Entity Framework configuration, visit http://go.microsoft.com/fwlink/?LinkID=237468 -->
<section name="entityFramework" type="System.Data.Entity.Internal.ConfigFile.EntityFrameworkSection, EntityFramework, Version=6.0.0.0, Culture=neutral, PublicKeyToken=b77a5c561934e089" requirePermission="false" />
<section name="log4net" type="log4net.Config.Log4NetConfigurationSectionHandler, log4net" />
</configSections>
<appSettings>
<add key="webpages:Version" value="3.0.0.0" />
<add key="webpages:Enabled" value="false" />
<add key="ClientValidationEnabled" value="true" />
<add key="UnobtrusiveJavaScriptEnabled" value="true" />
</appSettings>
<system.web>
<httpRuntime maxUrlLength="1000" maxQueryStringLength="10000" targetFramework="4.5.2" requestValidationMode="2.0" requestPathInvalidCharacters="" enableVersionHeader="false" relaxedUrlToFileSystemMapping="true" />
<compilation targetFramework="4.5.2" />
<httpCookies httpOnlyCookies="true" requireSSL="false" />
<trace writeToDiagnosticsTrace="false" enabled="false" pageOutput="false" localOnly="true" />
<authentication mode="Windows" />
<authorization>
<deny users="?" />
</authorization>
<customErrors mode="Off"></customErrors>
</system.web>
<system.webServer>
<handlers>
<remove name="WebDAV" />
<remove name="ExtensionlessUrlHandler-Integrated-4.0" />
<remove name="OPTIONSVerbHandler" />
<remove name="TRACEVerbHandler" />
<add name="ExtensionlessUrlHandler-Integrated-4.0" path="*." verb="*" type="System.Web.Handlers.TransferRequestHandler" preCondition="integratedMode,runtimeVersionv4.0" />
</handlers>
<httpCompression directory="[[REDACTED]]\IISContentCompression" minFileSizeForComp="1024">
<scheme name="gzip" dll="%Windir%\system32\inetsrv\gzip.dll" />
<dynamicTypes>
<add mimeType="text/*" enabled="true" />
<add mimeType="message/*" enabled="true" />
<add mimeType="application/javascript" enabled="true" />
<add mimeType="application/json" enabled="true" />
<add mimeType="*/*" enabled="false" />
</dynamicTypes>
<staticTypes>
<add mimeType="text/*" enabled="true" />
<add mimeType="message/*" enabled="true" />
<add mimeType="application/javascript" enabled="true" />
<add mimeType="application/json" enabled="true" />
<add mimeType="*/*" enabled="false" />
</staticTypes>
</httpCompression>
<httpErrors existingResponse="PassThrough" errorMode="Custom">
<!-- Catch IIS 404 error due to paths that exist but shouldn't be served (e.g. /controllers, /global.asax) or IIS request filtering (e.g. bin, web.config, app_code, app_globalresources, app_localresources, app_webreferences, app_data, app_browsers) -->
<remove statusCode="404" subStatusCode="-1" />
<error statusCode="404" subStatusCode="-1" path="/notfound" responseMode="ExecuteURL" />
<remove statusCode="500" subStatusCode="-1" />
<error statusCode="500" subStatusCode="-1" path="/error" responseMode="ExecuteURL" />
</httpErrors>
<urlCompression doStaticCompression="true" doDynamicCompression="true" />
<directoryBrowse enabled="false" />
<validation validateIntegratedModeConfiguration="false" />
<modules runAllManagedModulesForAllRequests="true">
<remove name="WebDAVModule" />
</modules>
<staticContent>
<!-- Set expire headers to 30 days for static content-->
<clientCache cacheControlMode="UseMaxAge" cacheControlMaxAge="30.00:00:00" />
<!-- use utf-8 encoding for anything served text/plain or text/html -->
<remove fileExtension=".css" />
<mimeMap fileExtension=".css" mimeType="text/css" />
<remove fileExtension=".js" />
<mimeMap fileExtension=".js" mimeType="text/javascript" />
<remove fileExtension=".json" />
<mimeMap fileExtension=".json" mimeType="application/json" />
<remove fileExtension=".rss" />
<mimeMap fileExtension=".rss" mimeType="application/rss+xml; charset=UTF-8" />
<remove fileExtension=".html" />
<mimeMap fileExtension=".html" mimeType="text/html; charset=UTF-8" />
<remove fileExtension=".xml" />
<mimeMap fileExtension=".xml" mimeType="application/xml; charset=UTF-8" />
<!-- HTML5 Audio/Video mime types-->
<remove fileExtension=".mp3" />
<mimeMap fileExtension=".mp3" mimeType="audio/mpeg" />
<remove fileExtension=".mp4" />
<mimeMap fileExtension=".mp4" mimeType="video/mp4" />
<remove fileExtension=".ogg" />
<mimeMap fileExtension=".ogg" mimeType="audio/ogg" />
<remove fileExtension=".ogv" />
<mimeMap fileExtension=".ogv" mimeType="video/ogg" />
<remove fileExtension=".webm" />
<mimeMap fileExtension=".webm" mimeType="video/webm" />
<!-- Proper svg serving. Required for svg webfonts on iPad -->
<remove fileExtension=".svg" />
<mimeMap fileExtension=".svg" mimeType="image/svg+xml" />
<remove fileExtension=".svgz" />
<mimeMap fileExtension=".svgz" mimeType="image/svg+xml" />
<!-- HTML4 Web font mime types -->
<!-- Remove default IIS mime type for .eot which is application/octet-stream -->
<remove fileExtension=".eot" />
<mimeMap fileExtension=".eot" mimeType="application/vnd.ms-fontobject" />
<remove fileExtension=".ttf" />
<mimeMap fileExtension=".ttf" mimeType="application/x-font-ttf" />
<remove fileExtension=".ttc" />
<mimeMap fileExtension=".ttc" mimeType="application/x-font-ttf" />
<remove fileExtension=".otf" />
<mimeMap fileExtension=".otf" mimeType="font/opentype" />
<remove fileExtension=".woff" />
<mimeMap fileExtension=".woff" mimeType="application/font-woff" />
<remove fileExtension=".crx" />
<mimeMap fileExtension=".crx" mimeType="application/x-chrome-extension" />
<remove fileExtension=".xpi" />
<mimeMap fileExtension=".xpi" mimeType="application/x-xpinstall" />
<remove fileExtension=".safariextz" />
<mimeMap fileExtension=".safariextz" mimeType="application/octet-stream" />
<!-- Flash Video mime types-->
<remove fileExtension=".flv" />
<mimeMap fileExtension=".flv" mimeType="video/x-flv" />
<remove fileExtension=".f4v" />
<mimeMap fileExtension=".f4v" mimeType="video/mp4" />
<!-- Assorted types -->
<remove fileExtension=".ico" />
<mimeMap fileExtension=".ico" mimeType="image/x-icon" />
<remove fileExtension=".webp" />
<mimeMap fileExtension=".webp" mimeType="image/webp" />
<remove fileExtension=".htc" />
<mimeMap fileExtension=".htc" mimeType="text/x-component" />
<remove fileExtension=".vcf" />
<mimeMap fileExtension=".vcf" mimeType="text/x-vcard" />
<remove fileExtension=".torrent" />
<mimeMap fileExtension=".torrent" mimeType="application/x-bittorrent" />
<remove fileExtension=".cur" />
<mimeMap fileExtension=".cur" mimeType="image/x-icon" />
<remove fileExtension=".webapp" />
<mimeMap fileExtension=".webapp" mimeType="application/x-web-app-manifest+json; charset=UTF-8" />
</staticContent>
<httpProtocol>
<customHeaders>
<remove name="X-Powered-By" />
<add name="X-Powered-By" value="[[REDACTED]]" />
<add name="X-UA-Compatible" value="IE=Edge,chrome=1" />
<add name="X-Content-Type-Options" value="nosniff" />
</customHeaders>
</httpProtocol>
<security>
<requestFiltering>
<requestLimits maxUrl="20000" maxQueryString="1073741824" maxAllowedContentLength="1073741824" />
</requestFiltering>
<authentication>
<windowsAuthentication enabled="true" />
<anonymousAuthentication enabled="false" />
</authentication>
</security>
</system.webServer>
<runtime>
<assemblyBinding xmlns="urn:schemas-microsoft-com:asm.v1">
<dependentAssembly>
<assemblyIdentity name="Newtonsoft.Json" culture="neutral" publicKeyToken="30ad4fe6b2a6aeed" />
<bindingRedirect oldVersion="0.0.0.0-10.0.0.0" newVersion="10.0.0.0" />
</dependentAssembly>
<dependentAssembly>
<assemblyIdentity name="System.Web.Optimization" publicKeyToken="31bf3856ad364e35" />
<bindingRedirect oldVersion="1.0.0.0-1.1.0.0" newVersion="1.1.0.0" />
</dependentAssembly>
<dependentAssembly>
<assemblyIdentity name="WebGrease" publicKeyToken="31bf3856ad364e35" />
<bindingRedirect oldVersion="0.0.0.0-1.6.5135.21930" newVersion="1.6.5135.21930" />
</dependentAssembly>
<dependentAssembly>
<assemblyIdentity name="System.Web.Helpers" publicKeyToken="31bf3856ad364e35" />
<bindingRedirect oldVersion="1.0.0.0-3.0.0.0" newVersion="3.0.0.0" />
</dependentAssembly>
<dependentAssembly>
<assemblyIdentity name="System.Web.Mvc" publicKeyToken="31bf3856ad364e35" />
<bindingRedirect oldVersion="1.0.0.0-5.2.3.0" newVersion="5.2.3.0" />
</dependentAssembly>
<dependentAssembly>
<assemblyIdentity name="System.Web.WebPages" publicKeyToken="31bf3856ad364e35" />
<bindingRedirect oldVersion="1.0.0.0-3.0.0.0" newVersion="3.0.0.0" />
</dependentAssembly>
<dependentAssembly>
<assemblyIdentity name="Antlr3.Runtime" publicKeyToken="eb42632606e9261f" culture="neutral" />
<bindingRedirect oldVersion="0.0.0.0-3.5.0.2" newVersion="3.5.0.2" />
</dependentAssembly>
</assemblyBinding>
</runtime>
<entityFramework>
<defaultConnectionFactory type="System.Data.Entity.Infrastructure.LocalDbConnectionFactory, EntityFramework">
<parameters>
<parameter value="mssqllocaldb" />
</parameters>
</defaultConnectionFactory>
<providers>
<provider invariantName="System.Data.SqlClient" type="System.Data.Entity.SqlServer.SqlProviderServices, EntityFramework.SqlServer" />
</providers>
</entityFramework>
<connectionStrings>
<add name="PP" connectionString="[[REDACTED]]" providerName="System.Data.SqlClient" />
</connectionStrings>
<location path="[[REDACTED]]">
<system.web>
<!--<authentication mode="None"/>-->
<authorization>
<allow users="?" />
</authorization>
</system.web>
<system.webServer>
<security>
<authentication>
<!--<anonymousAuthentication enabled="true" />-->
</authentication>
</security>
</system.webServer>
</location>
<log4net>
<root>
<level value="ALL" />
<appender-ref ref="ApplicationReportingDB" />
</root>
<appender name="ApplicationReportingDB" type="log4net.Appender.AdoNetAppender">
<bufferSize value="1" />
<connectionType value="System.Data.SqlClient.SqlConnection, System.Data, Version=1.0.3300.0, Culture=neutral, PublicKeyToken=b77a5c561934e089" />
<connectionString value="[[REDACTED]]" />
<commandText value="INSERT INTO ErrorLog ([Date],[Thread],[Level],[Logger],[Message],[Exception]) VALUES (@log_date, @thread, @log_level, @logger, @message, @exception)" />
<parameter>
<parameterName value="@log_date" />
<dbType value="DateTime" />
<layout type="log4net.Layout.RawTimeStampLayout" />
</parameter>
<parameter>
<parameterName value="@thread" />
<dbType value="String" />
<size value="255" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%thread" />
</layout>
</parameter>
<parameter>
<parameterName value="@log_level" />
<dbType value="String" />
<size value="50" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%level" />
</layout>
</parameter>
<parameter>
<parameterName value="@logger" />
<dbType value="String" />
<size value="255" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%logger" />
</layout>
</parameter>
<parameter>
<parameterName value="@message" />
<dbType value="String" />
<size value="4000" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%message" />
</layout>
</parameter>
<parameter>
<parameterName value="@exception" />
<dbType value="String" />
<size value="2000" />
<layout type="log4net.Layout.ExceptionLayout" />
</parameter>
<parameter>
<parameterName value="[[REDACTED]]" />
<dbType value="String" />
<size value="255" />
<layout type="log4net.Layout.PatternLayout">
<conversionPattern value="%property{CustomColumn}" />
</layout>
</parameter>
</appender>
</log4net>
<location path="[[REDACTED]]">
<system.web>
<authorization>
<allow users="?" />
</authorization>
</system.web>
<system.webServer>
<security>
<authentication>
<anonymousAuthentication enabled="true" />
</authentication>
</security>
</system.webServer>
</location>
</configuration>